Optimized protocol for DNA/RNA co-extraction from adults of Dirofilaria immitis
نویسندگان
چکیده
منابع مشابه
Feline Dirofilaria immitis infection
AffectedAnimals: Dogs and cats. Overall, cats are infected by heartworms much less commonly, even in areas in which heavily infected dogs are present. Male cats tend to be infected more commonly and have a larger worm burden than female cats, mostly because male cats spend more time roaming outside and have less resistance to infection than female cats. Cats that spend significant amounts of ti...
متن کاملMorphological redescription of Dirofilaria immitis.
Morphological descriptions of Dirofilaria immitis are scarce. For this reason, we carried out morphological studies using both light and scanning electron microscopy for this filaroid species. Morphometric and morphological data were compatible with previous descriptions of D. immitis , but several anatomical structures are described by scanning electron microscopy for the first time, such as d...
متن کاملGenetic polymorphism in Dirofilaria immitis.
Dirofilaria immitis is the causative agent of heartworm disease. Reports of macrocyclic lactone inefficacy prompted an investigation of genetic polymorphism in D. immitis. Currently, there is a lack of genetic information for this parasite. Information on baseline levels of genetic heterogeneity in the dog heartworm would have important implications for the possible development of macrocyclic l...
متن کاملPrevalence of Dirofilaria immitis in dogs from Hatay province, Turkey.
The present study was conducted to determine the prevalence of canine dirofilariosis in Hatay province, south of Turkey. A total of 269 blood samples were collected from owned dogs in this multi-centre survey between March and July 2006. Blood samples were examined by modified Knott and enzyme-linked immunosorbent assay (ELISA) techniques to detect circulating microfilariae and antigens of Diro...
متن کاملABC-B transporter genes in Dirofilaria immitis
Dirofilaria immitis is a filarial nematode causing infection and heartworm disease in dogs and other canids, cats, and occasionally in humans. Prevention with macrocyclic lactones (ML) is recommended during the mosquito transmission season. Recently, ML resistance has been reported. ABC-B transporter genes are thought to be involved in the mechanism of ML resistance in other nematodes. This stu...
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ژورنال
عنوان ژورنال: MethodsX
سال: 2019
ISSN: 2215-0161
DOI: 10.1016/j.mex.2019.10.023